Which of the following conditions is most likely to cause an enzyme to denature? High substrate concentration. High enzyme concentration. Extreme temperature or pH. Optimal temperature and pH.

## Step 1<br />Enzymes are biological catalysts that speed up chemical reactions in living organisms. They function best at an optimal temperature and pH. <br /><br />## Step 2<br />However, extreme temperature or pH can cause the enzyme to denature. Denaturation refers to the process where the protein loses its structure due to the disruption of weak bonds that hold the protein in its secondary and tertiary structure. <br /><br />## Step 3<br />This disruption of weak bonds can be caused by extreme temperature or pH, which can break the hydrogen bonds and other forces that hold the protein in its secondary and tertiary structure. <br /><br />## Step 4<br />High substrate concentration or high enzyme concentration does not cause denaturation. Instead, they can speed up the reaction rate until all the substrate is used up. <br /><br />## Step 5<br />Therefore, the most likely condition to cause an enzyme to denature is extreme temperature or pH.